Fungal peroxygenases and methods of application

US8367387B2 · kind B2 · utility

Assignee

• Novozymes A/S · DK

Inventors

• Marek Jan Pecyna · Bodenwerder, DE
• Kirk Matthew Schnorr · Paseo Los Muchachos, DK
• Rene Ullrich · Zittau, DE
• Katrin Scheibner · Jena, DE
• Martin Gunter Kluge · Zittau, DE
• Martin Hofrichter · Dresden, DE

Key dates

• Filing date: Mar 31, 2008
• Grant date: Feb 5, 2013
• Expiry date: Oct 15, 2028

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N9/0065
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention relates to polypeptides having peroxygenase activity and compositions comprising such polypeptides, their encoding polynucleotides, expression vectors and recombinant host cells comprising such polynucleotides or vectors, methods of producing the polypeptides, as well as methods of application and uses thereof, including a process for enzymatic, regioselective oxygenation of N-heterocycles of the general formula (I) to the corresponding N-oxides of the formula (II), by converting N-heterocycles of the formula (I) with a peroxidase polypeptide in the presence of at least one oxidizing agent in a one-stage reaction process.