Methods and means for removal of a selected DNA sequence
US8367890B2 · kind B2 · utility
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29Claims
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Key dates
| Filing date | Sep 20, 2007 |
| Grant date | Feb 5, 2013 |
| Priority date | — |
| Expiry date | Sep 10, 2029 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA01H1/045
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Alternative and/or improved methods are described for the exact removal of a selected subfragment from a DNA molecule by intrachromosomal recombination between two directly repeated DNA sequences using a rare-cleaving double stranded break inducing DNA endonuclease expressed under control of a micro-spore specific promoter. These methods can be applied for the exact exchange of a target DNA fragment for a DNA fragment of interest in plant cells and plants.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.