Method for production of separation media
US8372286B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jan 30, 2009 |
| Grant date | Feb 12, 2013 |
| Priority date | — |
| Expiry date | Dec 25, 2029 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01J2220/52
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
The present invention relates to a method for production of separation media using a so called Spinning Disc technology wherein the porosities of the beads are optimized in such a way that a desired biomolecule may be separated from a complex sample. The method comprises the following steps: a) feeding a 4-8% polysaccharide solution, which has a viscosity within 350-450 mPas, at 65-75° C. to one or more spinning discs at 3001-3010 rpm to form polysaccharide beads; b) capturing said formed polysaccharide beads in a capturing bath; wherein the porosity of the polysaccharide beads is controlled by varying the temperature of the capturing between 15 and 27° C., preferably between 17.5 and 24.6° C. The method yields porosities that prevent molecules larger than 150 000 g/mol to diffuse into the beads. The invention also relates to separation media produced by the method and use thereof for purification of biomolecules, in particular monoclonal antibodies.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.