Modular monolayer coatings for selective attachment of nanoparticles to biomolecules

US8372435B2 · kind B2 · utility

Assignee

• THE TRUSTEES OF PRINCETON UNIVERSITY · US

Inventors

• Jeffrey Schwartz · Princeton, US
• Christopher A. Traina · Napa, US
• Thomas J. Dennes · Parkesburg, US

Key dates

• Filing date: Jun 22, 2009
• Grant date: Feb 12, 2013
• Expiry date: Dec 23, 2030

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10T428/2991
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Nanoparticles are functionalized for use as bio-imaging probes using a novel, modular approach. Particle surface modification is based on a phosphonate monolayer platform on which was built a multi-segmented, multi-functional film: the first segment provided hydrolytic stability, the second aqueous suspendability, and the third, selectivity for cell attachment. In vitro imaging experiments visualized nanoparticle-cell surface binding. Peptide-derivatized nano-particles were not displaced from cells by soluble peptide. Methods for coating the host particles and use of rare earth ion-doped particles in imaging methods and photodynamic therapy methods are also disclosed.