High fidelity restriction endonucleases
US8372619B2 · kind B2 · utility
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8Claims
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Key dates
| Filing date | Jul 14, 2008 |
| Grant date | Feb 12, 2013 |
| Priority date | — |
| Expiry date | Apr 6, 2031 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/1086
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Compositions and methods are provided for enzymes with altered properties that involve a systematic approach to mutagenesis and a screening assay that permits selection of the desired proteins. Embodiments of the method are particularly suited for modifying specific properties of restriction endonucleases such as star activity. The compositions include restriction endonucleases with reduced star activity as defined by an overall fidelity index improvement factor.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.