Patent · US Active

Production of recombinant proteins by autoproteolytic cleavage of a fusion protein

US8372959B2 · kind B2 · utility

0Cited by

1References

18Claims

0Family size

Assignees

Sandoz AG · CH
BOEHRINGER INGELHEIM RCV GMBH & CO KG · AT

Inventors

Alois Jungbauer · Wien, AT
Rainer Hahn · Tübingen, DE
Anne Tscheliessnig · Ledenitzen, AT
Waltraud Kaar · Wien, AT

Key dates

Filing date	Jan 11, 2012
Grant date	Feb 12, 2013
Priority date	—
Expiry date	Jan 11, 2032

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12N15/62
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

Disclosed is a method for the production of a heterologous polypeptide of interest with a homogenous N-terminus, using a fusion polypeptide comprising the polypeptide of interest and N-terminally thereto a polypeptide exhibiting autoproteolytic function, said method comprises the steps of a) binding of the fusion polypeptide in a soluble, autoproteolytically inactive form by an affinity chromatography system, b) refolding of the fusion polypeptide, thereby activating the autoproteolytic function of the fusion polypeptide and causing cleavage of the heterologous polypeptide of interest, and c) subsequently eluting the heterologous polypeptide of interest, wherein the steps are conducted on one affinity chromatography system.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.