Viral expression plasmids for production of proteins, antibodies, enzymes, virus-like particles and for use in cell-based assays

US8377653B2 · kind B2 · utility

Assignee

• Icosagen Cell Factory OÜ · EE

Inventors

• Toomas Silla · Tartu, EE
• Ingrid Tagen · Tartu, EE
• Anne Kalling · Tartu, EE
• Radi Tegova · Tartu, EE
• Mart Ustav · Tartu, EE
• Tiiu Mandel · Tartu, EE
• Urve Toots · Tartu, EE
• Andres Tover · Tartu, EE
• Aare Abroi · Tartu, EE
• Ene Ustav · Tartu, EE
• Jelizaveta Geimanen · Tartu, EE
• Kadri Janikson · Tartu, EE

Key dates

• Filing date: Jul 7, 2010
• Grant date: Feb 19, 2013
• Expiry date: Sep 19, 2030

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2820/60
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

This disclosure shows that the EBV FR-element comprised of EBNA1 multimeric binding sites can provide the stable maintenance replication function to the mouse polyomavirus (PyV) core origin plasmids in the presence of BPV-1 E2 protein and PyV large T-antigen (LT).