Microorganisms for the production of 1,4-butanediol, 4-hydroxybutanal, 4-hydroxybutyryl-coa, putrescine and related compounds, and methods related thereto

US8377666B2 · kind B2 · utility

Assignee

• Genomatica, Inc. · US

Inventors

• Robert Haselbeck · San Diego, US
• John D. Trawick · San Diego, US
• Wei Niu · San Diego, US
• Anthony P. Burgard · Bellefonte, US

Key dates

• Filing date: Oct 13, 2010
• Grant date: Feb 19, 2013
• Expiry date: Oct 13, 2030

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12P19/40
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention provides non-naturally occurring microbial organisms comprising a 1,4-butanediol (BDO), 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine pathway comprising at least one exogenous nucleic acid encoding a BDO, 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine pathway enzyme expressed in a sufficient amount to produce BDO, 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine and further optimized for expression of BDO. The invention additionally provides methods of using such microbial organisms to produce BDO, 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine.