Patent · US Active

Microfluidic device having stable static gradient for analyzing chemotaxis

US8377685B2 · kind B2 · utility

5Cited by
1References
80Claims
0Family size

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Inventors

Key dates

Filing dateNov 7, 2008
Grant dateFeb 19, 2013
Priority date
Expiry dateSep 24, 2031

Classification

  • Technology area (CPC B)Performing Operations; Transporting
  • CPC primaryB01L2400/0472
  • WIPO fieldChemical engineering
  • WIPO sectorChemistry

Abstract

A microfluidic method and device for testing and analyzing chemotaxis by providing a stable, static fluid gradient. The device includes a sink reservoir for receiving biological cellular material and a source reservoir for receiving a chemoattractant. The biological cellular material migrates through a low fluid volume microfluidic gradient channel located between the source and sink reservoirs. The fluid in the gradient channel is static and stable due to a high fluid volume closed circuit bypass microfluidic channel also in fluid communication with the source and sink reservoirs, whereby the bypass channel relieves any pressure differential imparted across the gradient channel.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.