Monomethine dyes

US8378115B2 · kind B2 · utility

Assignee

• Thermo Fisher Scientific (Milwaukee) LLC · US

Inventors

• Charles K. Brush · Whitefish Bay, US
• Jianqin Liu · Beijing, CN
• Peter T. Czerney · Weimar, DE
• Matthias S. Wenzel · Jena, DE

Key dates

• Filing date: Sep 30, 2008
• Grant date: Feb 19, 2013
• Expiry date: Jan 24, 2029

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6827
• WIPO field: Organic fine chemistry
• WIPO sector: Chemistry

Abstract

Monomethine dyes that have no or minimal fluorescence in buffer or in the presence of single stranded DNA or RNA, but strongly fluoresce in the presence of double-stranded DNA. In one embodiment, the dye is useful in quantitative RT-PCR.