Assay for monitoring parathyroid hormone (PTH) variants by tandem mass spectrometry
US8383417B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Dec 22, 2009 |
| Grant date | Feb 26, 2013 |
| Priority date | — |
| Expiry date | Feb 15, 2030 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/24
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Methods are described for monitoring the amounts of PTH variants in a biological sample by digesting the sample to produce surrogate peptides specific to the targeted PTH variants, and detecting and quantifying the surrogate peptides by selective reaction monitoring (SRM) mass spectrometry, using a set of precursor-to-product ion transitions optimized for sensitivity and selectivity. The PTH variants, or a portion thereof, may be concentrated in the sample by means of immunoaffinity capture or other suitable technique. The mass spectrometric method described herein enables the concurrent measurement of peptides representative of a plurality of targeted PTH variants in a single assay.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.