DNA methylation detection methods
US8394585B2 · kind B2 · utility
3Cited by
0References
38Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jul 11, 2011 |
| Grant date | Mar 12, 2013 |
| Priority date | — |
| Expiry date | Jul 11, 2031 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6827
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present teachings provide DNA methylation quantification methods that avoid bisulfite treatment of DNA. Methylation-specific binding proteins (MeDNA binding proteins) and non-methylation specific binding proteins (non-MeDNA binding proteins) are employed in various embodiments to modulate the accessibility of nucleic acids to primer extension reactions. After selectively removing the target nucleic acids, the extension products can be analyzed and methylation quantitated. In some embodiments, the analysis comprises real-time PCR.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.