Methods to fix and detect nucleic acids

US8394588B2 · kind B2 · utility

Assignee

• The Rockefeller University · US

Inventors

• Thomas Tuschl · Oettingen, DE
• John Pena · New York, US
• Cherin Sohn · New York, US
• Sara Hakim · New York, US
• Janos Ludwig · Göttingen, DE
• Pavol Cekan · Gaithersburg, US

Key dates

• Filing date: Mar 10, 2010
• Grant date: Mar 12, 2013
• Expiry date: Oct 1, 2030

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6841
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

In one aspect, the invention relates to a method for fixing a short nucleic acid in a biological sample. In another aspect, the invention relates to a method for detecting a target short nucleic acid in a biological sample. The method includes contacting the biological sample with an aldehyde-containing fixative, and subsequently contacting the sample with a water-soluble carbodiimide. In a further aspect, the invention relates to a kit for fixing a short nucleic acid in a biological sample. The kit includes a support substrate for holding the sample; an aldehyde-containing fixative; and a water-soluble carbodiimide.