Simultaneous detection of metabolic enzyme activity and metabolite levels
US8399186B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Jan 24, 2012 |
| Grant date | Mar 19, 2013 |
| Priority date | — |
| Expiry date | Jan 24, 2032 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2800/04
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Provided are methods for detecting a metabolic disorder in an individual using mass spectrometry. One method involves (a) contacting a sample containing (i) a metabolically indicative enzyme and (ii) a metabolic analyte, with a substrate for the enzyme to produce a reaction admixture, under conditions wherein the enzyme is capable of acting on a corresponding substrate to generate a product, and wherein a protease inhibitor is present; (b) contacting the reaction admixture with a reagent that inhibits the ability of the enzyme to act on a corresponding substrate, wherein the metabolic analyte and the product are soluble in the reagent; to produce a test sample and (c) determining the presence or amount of the metabolic analyte and the product contained in the test sample using mass spectrometry, wherein a determined presence or amount of the metabolic analyte and the product correlates with presence or absence of the metabolic disorder.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.