Isolation of allergen-specific immunoglobulin genes from human B-cells from atopy sufferers

US8399200B2 · kind B2 · utility

Assignee

• Fraunhofer-Gesellschaft zur förderung der angewandten Forschung e.V. · DE

Inventors

• Gernot Achatz · Salzburg, AT
• Michael Huhn · Leverkusen, DE
• Rainer Fischer · Monschau, DE
• Fatima Ferreira · Salzburg, AT
• Elke Luger · Wels, AT
• Michael Stöcker · Aachen, DE
• Stefan Barth · Jena, DE
• Torsten Klockenbring · Aachen, DE

Key dates

• Filing date: May 25, 2005
• Grant date: Mar 19, 2013
• Expiry date: Jan 13, 2029

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2317/622
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A process is disclosed which enables the establishing of comprehensive immunoglobulin-specific reaction profiles of subjects with disorders of the immune function by the individual V gene repertoire of Ig-expressing B cells. The process comprises the isolation of B cells from body fluids, the isolation of individual B cells and their genetic material, the amplification of nucleic acids coding the variable regions of an antibody expressed by a B cell, the recombinant preparation of antibodies by expressing the amplificates, and the determination of the binding capability of the recombinant antibody to antigens.