Bioluminescent assays utilising secreted luciferases
US8399212B2 · kind B2 · utility
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11Claims
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Key dates
| Filing date | Dec 21, 2007 |
| Grant date | Mar 19, 2013 |
| Priority date | — |
| Expiry date | Nov 1, 2028 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/182
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Disclosed herein are methods for determining the amount or activity of one or more luciferases and methods for measuring the luminescent signal generated by one or more luciferases in a sample, the methods comprising incubating the sample with a reactive substrate(s) of the luciferase(s) to be analysed and a reducing agent to inactivate a first luciferase, wherein the first luciferase, in its native form, is a secreted luciferase.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.