High density growth of T7 expression strains with auto-induction option
US8399217B2 · kind B2 · utility
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25Claims
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Key dates
| Filing date | Apr 27, 2007 |
| Grant date | Mar 19, 2013 |
| Priority date | — |
| Expiry date | Mar 10, 2030 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12P19/34
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for promoting and suppressing auto-induction of transcription of a cloned gene 1 of bacteriophage T7 in cultures of bacterial cells grown batchwise is disclosed. The transcription is under the control of a promoter whose activity can be induced by an exogenous inducer whose ability to induce said promoter is dependent on the metabolic state of said bacterial cells.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.