Methods for detection and quantitation of small RNAs
US8399221B2 · kind B2 · utility
2Cited by
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8Claims
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Key dates
| Filing date | Nov 4, 2008 |
| Grant date | Mar 19, 2013 |
| Priority date | — |
| Expiry date | Nov 4, 2028 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6844
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Improved methods that increase the specificity and sensitivity of detection of small RNAs, including miRNAs, using oligonucleotide primers and nucleic acid amplification, are provided. Reaction conditions that result in preferential decrease in cDNA synthesis of RNAs other than the small RNA molecules targeted for detection during miRNA tailing and reverse transcription reactions are described. Using these reaction conditions greater sensitivity and specificity of amplification of small RNAs including miRNAs is achieved.
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