Patent · US Active

Compositions and methods for detecting small RNAs, and uses thereof

US8399222B2 · kind B2 · utility

26Cited by
62References
45Claims
0Family size

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Key dates

Filing dateNov 25, 2009
Grant dateMar 19, 2013
Priority date—
Expiry dateSep 4, 2031

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12Q2600/178
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

Compositions and methods are provided for the detection of small RNA target nucleic acids, preferably miRNA target nucleic acids, wherein the compositions and methods provide for sensitive and specific detection of the target nucleic acids. The compositions and methods include using one or more of a first amplification oligomer that is preferably an extender primer, a target capture oligomer that is preferably at least partially double stranded, a promoter primer/provider, a reverse primer that is preferably a universal primer and a detection probe. The compositions and methods are useful for diagnostics, prognostics, monitoring the effectiveness of treatment and/or determining a treatment.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.