Purification of factor VIII using a mixed-mode or multimodal resin
US8399620B2 · kind B2 · utility
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16Claims
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Key dates
| Filing date | Jul 11, 2008 |
| Grant date | Mar 19, 2013 |
| Priority date | — |
| Expiry date | Sep 19, 2029 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K14/755
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for purifying a recombinant protein using a multimodal or mixed mode resin containing ligands which comprise a hydrophobic part and a negatively charged part is described. The invention is advantageous in that it is a single step chromatographic process which does not require adjustment of pH or conductivity during loading step and results in high yield and potency. The process is used for the purification of recombinant compositions of coagulation factor, particularly recombinant Factor VIII.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.