Patent · US Active

Bacillus licheniformis and method for detoxification of zearalenone

US8404477B2 · kind B2 · utility

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7Claims
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Key dates

Filing dateMay 28, 2010
Grant dateMar 26, 2013
Priority date
Expiry dateMay 28, 2030

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12R2001/10
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

The present invention discloses an isolated pure culture of a novel strain CK1 of Bacillus licheniformis, DSM 025954, capable of detoxification of zearalenone (ZEN). Using physiological, biochemical, morphological identification and 16S rRNA gene sequence analysis methods, the strain CK1 was identified as Bacillus licheniformis. Through extracellular xylanase, CMCase protease assays and evaluations for zearalenone detoxification, the strain CK1 strain was identified to possess good ZEN-detoxifying ability, to be non-hemolytic, non-enterotoxin producing, and displayed high levels of extracellular xylanase, cellulase, and protease activities. Accordingly, Bacillus licheniformis CK1 can be applied as food and feed supplement for bio-detoxification of ZEN.

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