Methods and intermediates for chemical synthesis of polypeptides and proteins
US8404804B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Aug 27, 2008 |
| Grant date | Mar 26, 2013 |
| Priority date | — |
| Expiry date | Jul 26, 2029 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K5/0821
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to methods and intermediates for chemical synthesis of polypeptides and proteins, and more particularly to methods and intermediates for chemically ligating a peptide fragment containing N-terminal N-methyl-cysteine (SEQ ID NO: 1) with another peptide fragment having C-terminal thioester to generate a β-(methylamino)-thioester intermediate that spontaneously rearranges to form an amide bond. Furthermore, the invention relates to methods of converting N-methyl-thiazolidine to N-methyl-cysteine (SEQ ID NO: 1) of polypeptides and proteins. The invention also relates to methods of synthesizing peptide-thioester from peptide-acid fluoride.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.