Blood and cell analysis using an imaging flow cytometer

US8406498B2 · kind B2 · utility

Assignee

• Amnis Corporation · US

Inventors

• William E. Ortyn · Bainbridge Island, US
• David A. Basiji · Seattle, US
• Philip J. Morrissey · Bellevue, US
• Thaddeus C. George · Seattle, US
• Brian Hall · Seattle, US
• Cathleen Zimmerman · Bainbridge Island, US
• David Perry · Woodinville, US

Key dates

• Filing date: Dec 4, 2009
• Grant date: Mar 26, 2013
• Expiry date: Jul 4, 2031

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2015/1488
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Multimodal or multispectral images of cells comprising a population of cells are simultaneously collected. Photometric and/or morphometric image features identifiable in the images are used to identify differences between first and second populations of cells. The differences can include changes in a relative percentage of different cell types in each population, or a change in a first type of cell present in the first population of cells and the same type of cell in the second population of cells. The changes may be indicative of a disease state, indicative of a relative effectiveness of a therapy, or indicative of a health of the person from whom the cells populations were obtained.