Method of amplification of GC-rich DNA templates
US8409805B2 · kind B2 · utility
5Cited by
14References
27Claims
0Family size
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Key dates
| Filing date | Feb 13, 2009 |
| Grant date | Apr 2, 2013 |
| Priority date | — |
| Expiry date | Feb 13, 2029 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/686
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods are provided for increasing the processivity of DNA polymerases on GC-rich templates. The methods relate to providing enhancers and biased ratios of dNTPs, and may be used in DNA amplification reactions. The methods are useful for detecting genotypes associated with GC-rich repeats, including Fragile X Syndrome.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.