Method of diagnosis and kit therefor

US8409818B2 · kind B2 · utility

Assignee

• The MacFarlane Burnet Institute for Medical Research and Public Health Ltd · AU

Inventors

• David Andrew Anderson · Brunswick, AU
• Robyn Elizabeth Lloyd · Brisbane, AU
• Suzanne Crowe · Melbourne, AU
• Mary Louise Garcia · Melbourne, AU
• Alan Lee Landay · Melbourne, AU

Key dates

• Filing date: Sep 28, 2007
• Grant date: Apr 2, 2013
• Expiry date: Oct 16, 2029

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10T436/25375
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The invention provides kits and methods for detecting or monitoring the number of cells in sample. The cell comprises a cell surface associated protein (CSAP) comprising a cytoplasmic (cytosolic) and an extracellular (ecto) domain. The kit comprises: (i) a chromatographic device; and (ii) a CSAP-binding agent. The method comprises: (i) optionally contacting the sample with an agent capable of lysing or permeabilizing CSAP bearing cells; (ii) contacting the sample with a CSAP-binding agent that binds to the cytoplasmic domain of the CSAP; and (iii) directly or indirectly evaluating the level or presence of bound CSAP in the sample.