Methods of unidirectional, site-specific integration into a genome, compositions and kits for practicing the same
US8420395B2 · kind B2 · utility
Assignees
Inventor
Key dates
| Filing date | Dec 3, 2004 |
| Grant date | Apr 16, 2013 |
| Priority date | — |
| Expiry date | Jun 2, 2026 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY02A40/146
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The subject invention provides a unidirectional site-specific integration system for integrating a nucleic acid into the genome of a target cell. The provided systems include a (1) a mutant, unidirectional site specific integrase, which can be provided by an integrase vector encoding the mutant integrase and (2) a targeting vector that includes: (a) a nucleic acid to be integrated; and (b) a vector attachment site, where the targeting vector attachment site serves as a substrate for the mutant, unidirectional site-specific integrase. In using the subject systems for site-specific integration, the targeting vector and integrase are introduced into the target cell and the cell is maintained under conditions sufficient to provide for site-specific integration of the nucleic acid into the target cell genome via a recombination event mediated by the site-specific integrase. Also provided are kits that include the subject systems. The subjects systems, methods and kits find use in a variety of different applications, several representative ones of which are described in detail as well.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.