Patent · US Active

Quantitative amplification with a labeled probe and 3′ to 5′ exonuclease activity

US8426132B2 · kind B2 · utility

10Cited by

31References

25Claims

0Family size

Assignee

Bio-Rad Laboratories, Inc. · US

Inventors

Bin Li · Columbus, US
Lei Xi · Zhengzhou, CN
Yan Wang · San Diego, US
Peter Vander Horn · Foster City, US

Key dates

Filing date	Nov 3, 2008
Grant date	Apr 23, 2013
Priority date	—
Expiry date	Jun 2, 2029

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12Q1/6851
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

This invention provides methods and kits for performing a quantitative amplification reaction. The method employs a polymerase enzyme and an enzyme having a 3′ to 5′ exonuclease activity that cleaves the 3′ oligonucleotide of the probe.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.