Meganuclease variants cleaving a DNA target sequence from the mouse ROSA26 locus and uses thereof
US8426177B2 · kind B2 · utility
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Key dates
| Filing date | Jun 6, 2008 |
| Grant date | Apr 23, 2013 |
| Priority date | — |
| Expiry date | Apr 24, 2029 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA01K2227/105
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
An I-CreI variant, wherein one of the two I-CreI monomers has at least two substitutions, one in each of the two functional subdomains of the LAGLIDADG (SEQ ID NO: 150) core domain situated respectively from positions 26 to 40 and 44 to 77 of I-CreI, said variant being able to cleave a DNA target sequence from the mouse ROSA26 locus. Use of said variant and derived products for the engineering of transgenic mice and recombinant mouse cell lines expressing an heterologous protein of interest.
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