Method for selective electrofusion of at least two fusion partners having cell-like membranes
US8426205B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jul 23, 2012 |
| Grant date | Apr 23, 2013 |
| Priority date | — |
| Expiry date | Jul 23, 2032 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N13/00
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Disclosed is a method for selective electrofusion of at least two fusion partners having cell-like membranes and cellular or subcellular dimensions, comprising the following steps: A) the fusion partners are brought into contact with each other and B) an electrical field of a strength sufficient to obtain fusion and highly focused on the fusion partners is applied. The fusion partners are independently selected from the group consisting of a single cell, a liposome, a proteoliposome, a synthetic vesicle, an egg cell, an enucleated egg cell, a sperm cell at any development stage and a plant protoplast.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.