Basic protein purification tags from thermophilic bacteria
US8426566B2 · kind B2 · utility
1Cited by
5References
13Claims
0Family size
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Key dates
| Filing date | Dec 1, 2011 |
| Grant date | Apr 23, 2013 |
| Priority date | — |
| Expiry date | Dec 1, 2031 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/20
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention is related to a method for purification of recombinant proteins using highly basic proteins from thermophilic bacteria as purification tags for use in a cation-exchange chromatography purification step. The basic proteins may be ribosomal proteins. The recombinant proteins are expressed in eukaryotic or prokaryotic host cells. The purification tag will typically have a pl above about 9 and comprise from about 15 to about 250 amino acid residues.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.