Amine-N-oxide redox indicators for fluorimetric determination of analytes

US8431410B2 · kind B2 · utility

Assignee

• Roche Diagnostics Operations, Inc. · US

Inventors

• Carina Horn · Biblis, DE
• Joachim Hoenes · Zwingenberg, DE
• Jurgen Spinke · Lorsch, DE

Key dates

• Filing date: Apr 9, 2010
• Grant date: Apr 30, 2013
• Expiry date: Jun 17, 2031

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10T436/144444
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A reagent for detecting an analyte by a redox reaction and a fluorimetric determination is disclosed. The reagent comprises a compound of formula (I):wherein R1 and R2 are each independently selected from R, (CH2CH2O)mR, COR, COOR, and OCOR; each R3 is independently selected from NO2, CN, R, OR, OCOR, COOR, SR, and halogen; R is H or C1-C4 alkyl, where alkyl is optionally substituted with one or more functional group independently selected from the group consisting of halogen, OR, SR, NR2, COOR, CONR2, SO3R and salts thereof, and PO(OR)3 and salts thereof; m is an integer from 1 to 20; and n is 1, 2, or 3. The reagent may further comprise an enzyme or a coenzyme for reducing or oxidizing an analyte.