Compositions and methods for modification of biomolecules

US8431558B2 · kind B2 · utility

Assignee

• The Regents of the University of California · US

Inventors

• Carolyn R. Bertozzi · Berkeley, US
• Nicholas J. Agard · San Francisco, US
• Jennifer A. Prescher · Berkeley, US
• Jeremy Michael Baskin · Ithaca, US
• Ellen May Sletten · Los Angeles, US

Key dates

• Filing date: Mar 14, 2008
• Grant date: Apr 30, 2013
• Expiry date: Mar 30, 2031

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07C2601/18
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The present invention provides modified cycloalkyne compounds; and method of use of such compounds in modifying biomolecules. The present invention features a cycloaddition reaction that can be carried out under physiological conditions. In general, the invention involves reacting a modified cycloalkyne with an azide moiety on a target biomolecule, generating a covalently modified biomolecule. The selectivity of the reaction and its compatibility with aqueous environments provide for its application in vivo (e.g., on the cell surface or intracellularly) and in vitro (e.g., synthesis of peptides and other polymers, production of modified (e.g., labeled) amino acids).