Cryopreservation of haptenized tumor cells
US8435784B2 · kind B2 · utility
Inventors
Key dates
| Filing date | Jun 13, 2007 |
| Grant date | May 7, 2013 |
| Priority date | — |
| Expiry date | May 16, 2030 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA61P35/00
- WIPO fieldBasic materials chemistry
- WIPO sectorChemistry
Abstract
A method of preserving haptenized tumor cells is described. The method employs a freezing medium containing an effective amount of sucrose and human serum albumin in an isotonic buffered saline solution. Cryogenically preserving haptenized cells in such a medium has been found to maintain the integrity of the tumor cells during storage. The haptenized tumor cells also retain cell-associated antigens and haptens, and are as immunogenic, i.e., capable of inducing immunotherapeutic response, as fresh vaccine in a mouse model of metastatic disease. In a specific embodiment, haptenized cells are exposed to a solution of 8% sucrose, 10% human serum albumin in Hank's buffered solution, and then frozen to −80° C. overnight and then stored in a liquid nitrogen freezer. Methods of storing haptenized tumor cells and compositions are also provided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.