Isolated DNA fragment of the SPARC human promoter and its use for driving the expression of an heterologous gene in tumor cells
US8436160B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Apr 26, 2007 |
| Grant date | May 7, 2013 |
| Priority date | — |
| Expiry date | Oct 4, 2029 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA61P43/00
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
An isolated DNA sequence corresponding to a region of the SPARC gene human promoter from base pair −513 to base pair +35 capable of driving the expression of a heterologous gene of interest, that can be associated to any other promoter sequence, such as radiation responsive sequence, hypoxia responsive sequence and free-radical responsive sequence. The invention also provides constructs and DNA recombinant expression viral vectors, comprising the isolated sequence of the SPARC gene human promoter and at least one heterologous gene operably linked thereto, wherein the promoter sequence drives the expression of the at least one heterologous gene in tumor cells. Pharmaceutical compositions and a method for treating tumors are also provided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.