Method of conducting a droplet based enzymatic assay
US8440392B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Mar 23, 2008 |
| Grant date | May 14, 2013 |
| Priority date | — |
| Expiry date | Aug 15, 2028 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2800/042
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method of conducting a droplet-based enzymatic assay is provided. The method generally makes use of a droplet actuator. A droplet comprising an enzyme of interest is provided on the droplet actuator along with a droplet comprising a substrate which is potentially modified in the presence of the enzyme. The method involves executing droplet operations on the droplet actuator to combine the droplets, thereby yielding an assay droplet, and detecting modification of the substrate by the enzyme in the assay droplet on the droplet actuator. The enzyme of interest may, for example, be a potentially mutated or improperly folded enzyme exhibiting altered enzyme activity as compared to a corresponding normal enzyme.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.