Diagnostic test for the detection of a molecule or drug in whole blood
US8440416B2 · kind B2 · utility
0Cited by
32References
25Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jul 12, 2012 |
| Grant date | May 14, 2013 |
| Priority date | — |
| Expiry date | Jul 12, 2032 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/9493
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The invention provides methods of preparing a test sample for use in an assay for detecting an analyte bound by an intracellular ligand. The methods typically involve contacting the test sample with an assay reagent comprising: a lysis reagent; and a protease that has proteolytic activity for said intracellular ligand; to form a mixture compatible for use in an immunoassay without subsequent extraction steps. Other aspects of the invention include related immunoassays and test kits.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.