Method for assaying compounds or agents for ability to displace potent ligands of hematopoietic prostaglandin D synthase

US8440417B2 · kind B2 · utility

Assignee

• CAYMAN CHEMICAL COMPANY, INC. · US

Inventors

• Nisha Palackal · White Plains, US
• Jeffrey Keith Johnson · Ann Arbor, US
• Karie L. McGowan · Manchester, US
• Kirk W. Maxey · Ann Arbor, US
• Gregory William Endres · Saline, US

Key dates

• Filing date: May 13, 2009
• Grant date: May 14, 2013
• Expiry date: Apr 7, 2031

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10T436/10
• WIPO field: Organic fine chemistry
• WIPO sector: Chemistry

Abstract

An exemplary embodiment may be directed to a fluorescence polarization assay that screens compounds or agents for their affinity to hematopoietic prostaglandin D synthase (H-PGDS) based on their ability to displace a fluorophore-containing detection analyte bound to an enzyme comprising the primary amino acid sequence of H-PGDS. Another exemplary embodiment utilizes an enzyme having a maltose binding protein amino-acid sequence fused with an N-terminus of the enzyme.