Multiple-photon excitation light sheet illumination microscope
US8441633B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Oct 29, 2010 |
| Grant date | May 14, 2013 |
| Priority date | — |
| Expiry date | Mar 3, 2031 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2021/655
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
An apparatus for and method of performing multi-photon light sheet microscopy (MP-LISH), combining multi-photon excited fluorescence with the orthogonal illumination of light sheet microscopy are provided. With live imaging of whole Drosophila and zebrafish embryos, the high performance of MP-LISH compared to current state-of-the-art imaging techniques in maintaining good signal and high spatial resolution deep inside biological tissues (two times deeper than one-photon light sheet microscopy), in acquisition speed (more than one order of magnitude faster than conventional two-photon laser scanning microscopy), and in low phototoxicity are demonstrated. The inherent multi-modality of this new imaging technique is also demonstrated second harmonic generation light sheet microscopy to detect collagen in mouse tail tissue. Together, these properties create the potential for a wide range of applications for MP-LISH in 4D imaging of live biological systems.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.