Patent · US Active

Hybrid superparamagnetic iron oxide nanoparticles and polyethylenimine as a magnetoplex for gene transfection

US8445025B2 · kind B2 · utility

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4Claims
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Key dates

Filing dateMar 29, 2011
Grant dateMay 21, 2013
Priority date—
Expiry dateMar 29, 2031

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12N15/85
  • WIPO fieldPharmaceuticals
  • WIPO sectorChemistry

Abstract

Disclosed are the nanoparticle and the method for the same, and the preparing method includes steps of mixing polyethylenimine (PEI) with the poly(acrylic acid)-bound iron oxide (PAAIO) to form a PEI-PAAIO polyelectrolyte complex (PEC) and mixing the PEI-PAAIO PEC with genetic material such as plasmid DNA to form the PEI-PAAIO/pDNA magnetic nanoparticle. The PEI-PAAIO/pDNA magnetoplex is highly water dispersible and suitable for long term storage, shows superparamagnetism, low cytotoxicity, high stability and nice transfection efficiency, and thus the PEI-PAAIO PEC can replace PEI as a non-viral gene vector.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.