Patent · US Active

Rationally-designed single-chain meganucleases with non-palindromic recognition sequences

US8445251B2 · kind B2 · utility

31Cited by
17References
1Claims
0Family size

Assignee

Inventors

Key dates

Filing dateApr 30, 2010
Grant dateMay 21, 2013
Priority date
Expiry dateJun 23, 2030

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12Y301/04
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

Disclosed are rationally-designed, non-naturally-occurring meganucleases in which a pair of enzyme subunits having specificity for different recognition sequence half-sites are joined into a single polypeptide to form a functional heterodimer with a non-palindromic recognition sequence. The invention also relates to methods of producing such meganucleases, and methods of producing recombinant nucleic acids and organisms using such meganucleases.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.