Patent · US Active

B12-dependent dehydratases with improved reaction kinetics

US8445659B2 · kind B2 · utility

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2References

4Claims

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Assignee

E.I. DU PONT DE NEMOURS AND COMPANY · US

Inventors

Katharine J. Gibson · Wilmington, US
Der-Ing Liao · Wilmington, US
Xiao-Song Tang · Hockessin, US

Key dates

Filing date	Aug 16, 2010
Grant date	May 21, 2013
Priority date	—
Expiry date	Apr 14, 2031

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12N9/88
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

Sequences of B12-dependent dehydratases with improved reaction kinetics are presented. Use of these B12-dependent dehydratases reduce the rate of the enzyme's suicide inactivation in the presence of glycerol and 1,3-propanediol. The enzymes were created using error-prone PCR and oligonucleotide-directed mutagenesis to target the DhaB1 gene, which encodes the α-subunit of glycerol dehydratase. Mutants with improved reaction kinetics were rapidly identified using high throughput assays.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.