Method of detection of microorganisms with enhanced bacteriophage amplification

US8455186B2 · kind B2 · utility

Assignee

• MicroPhage™ Incorporated · US

Inventors

• Jonathan Smith · Shaker Heights, US
• Jon C. Rees · Snellville, US
• Duane Bush · Fort Collins, US
• Breanna Leigh Dreiling · Longmont, US
• Maria Izzo · Longmont, US
• Breanna Christine Smith · Lyons, US
• Bernard Sportmann · Longmont, US
• Tiffany Steinmark · Golden, US
• Richard Proctor · Litchfield, US

Key dates

• Filing date: Jun 13, 2008
• Grant date: Jun 4, 2013
• Expiry date: Aug 6, 2029

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/56911
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A method of determining the presence or absence of a target microorganism in a sample to be tested, the method comprising: combining with the sample an amount of bacteriophage capable of attaching to the target microorganism to create a bacteriophage exposed sample, and a substance which enhances bacteriophage amplification or sensitivity; providing conditions to the bacteriophage-exposed sample sufficient to allow the bacteriophage to infect the microorganism; and assaying the bacteriophage-exposed sample to detect the presence or absence of a bacteriophage marker to determine the presence or absence of the target microorganism.