Compositions and methods for nucleic acid sequencing

US8455193B2 · kind B2 · utility

Assignee

• PACIFIC BIOSCIENCES OF CALIFORNIA, INC. · US

Inventors

• Kevin Travers · Menlo Park, US
• Geoff Otto · Brookline, US
• Stephen Turner · Menlo Park, US
• Cheryl Heiner · La Honda, US
• Congcong Ma · Foster City, US

Key dates

• Filing date: Feb 23, 2012
• Grant date: Jun 4, 2013
• Expiry date: Feb 23, 2032

Classification

• Technology area (CPC G): Physics
• CPC primary: G16B30/10
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Compositions and methods for nucleic acid sequencing include template constructs that comprise double stranded portions in a partially or completely contiguous constructs, to provide for redundant sequence determination through one or both of sequencing sense and antisense strands, and iteratively sequencing the entire construct multiple times. Additional sequence components are also optionally included within such template constructs. Methods are also provided for the use and preparation of these constructs as well as sequencing compositions for their application.