Method to facilitate directed delivery and electroporation using a charged stream

US8455228B2 · kind B2 · utility

Assignee

• University of South Florida · US

Inventors

• Mark J. Jaroszeski · Tampa, US
• Gabriel A. Lopez-Diaz · Wesley Chapel, US
• Richard J. Connolly · Riverview, US
• Andrew M. Hoff · State College, US

Key dates

• Filing date: Sep 10, 2008
• Grant date: Jun 4, 2013
• Expiry date: Mar 24, 2031

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12M35/02
• WIPO field: Medical technology
• WIPO sector: Instruments

Abstract

A method for facilitating a delivery of a molecule into an interior space of a cell includes the steps of introducing a molecule into a biological structure comprising a cell and applying a substantially continuous low-level electric field, in the form of non-thermal plasma (ionized gas) generated by a direct current voltage applied to an electrode, to the molecule and biological structure. The field is applied for a duration sufficient to effect a change in porosity the cell of the biological structure sufficient to facilitate an entry of a desired molecule into an interior thereof.