Yeast organism producing isobutanol at a high yield

US8455239B2 · kind B2 · utility

Assignee

• Gevo, Inc. · US

Inventors

• Reid M. Renny Feldman · San Marino, US
• Uvini Gunawardena · Irvine, US
• Jun Urano · Irvine, US
• Peter Meinhold · Santa Monica, US
• Aristos Aristidou · Highlands Ranch, US
• Catherine Asleson Dundon · Englewood, US
• Christopher Smith · Woodstock, US

Key dates

• Filing date: Jun 22, 2010
• Grant date: Jun 4, 2013
• Expiry date: Jun 22, 2030

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y02E50/10
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention provides recombinant mircoorganisms comprising an isobutanol producing metabolic pathway and methods of using said recombinant microorganisms to produce isobutanol. In various aspects of the invention, the recombinant microorganisms may comprise a modification resulting in the reduction of pyruvate decarboxylase and/or glycerol-3-phosphate dehydrogenase activity. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the Saccharomyces clade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.