Method of imaging with fluorescent microspheres

US8460638B2 · kind B2 · utility

Assignee

• INSTITUTE OF PROCESS ENGINEERING, CHINESE ACADEMY OF SCIENCES · CN

Inventors

• Guanghui Ma · Beijing, CN
• Zhiguo Su · Beijing, CN
• Wei Wei · Beijing, CN
• Lianyan Wang · Beijing, CN
• Qiang Wei · Guiyang, CN

Key dates

• Filing date: Sep 13, 2007
• Grant date: Jun 11, 2013
• Expiry date: Nov 9, 2029

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/533
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A method of fluorescence imaging is disclosed that uses a non-labeled fluorescent microsphere comprising a primary amine polymer, wherein the microsphere does not contain an additional fluorescent substance, and wherein at least one primary amine group in the primary amine polymer has the structure of —N═R—, in which R is a residue after the reaction of an aldehyde-type cross-linking substance, and the other primary amine groups have the structure of —NH2. The fluorescence color of the microspheres can be adjusted by selecting different aldehyde-type cross-linking substances or combinations, and the fluorescence intensity of the microspheres can be controlled not only by the addition of the aldehyde-type cross-linking substances and the time of the cross-linking reaction, but also by adjusting the diameters of the microspheres.