Patent · US Active

Recombinase polymerase amplification

US8460875B2 · kind B2 · utility

45Cited by
34References
21Claims
0Family size

Assignee

Inventors

Key dates

Filing dateJul 28, 2011
Grant dateJun 11, 2013
Priority date—
Expiry dateJul 28, 2031

Classification

  • Technology area (CPC G)Physics
  • CPC primaryG06F16/9574
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

This disclosure provides methods for Recombinase-Polymerase Amplification (RPA) of a target DNA. The methods exploit the properties of the bacterial RecA and related proteins to invade double-stranded DNA with single stranded homologous DNA permitting sequence specific priming of DNA polymerase reactions. The methods have the advantage of not requiring thermocycling or thermophilic enzymes. Further, the methods allow amplification of DNA up to hundreds of megabases in length.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.