Sensitive and rapid determination of antimicrobial susceptibility
US8460887B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Feb 8, 2010 |
| Grant date | Jun 11, 2013 |
| Priority date | — |
| Expiry date | Jun 16, 2030 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB82Y15/00
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention relates to moving microorganisms to a surface, where they are grown in the presence and absence of antimicrobials, and by monitoring the growth of the microorganisms over time in the two conditions, their susceptibility to the antimicrobials can be determined. The microorganisms can be moved to the surface through electrophoresis, centrifugation or filtration. When the movement involves electrophoresis, the presence of oxidizing and reducing reagents lowers the voltage at which electrophoretic force can be generated and allows a broader range of means by which the target can be detected. Monitoring can comprise optical detection, and most conveniently includes the detection of individual microorganisms. The microorganisms can be stained in order to give information about their response to antimicrobials.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.