Patent · US Active

Promoterless cassettes for expression of alpha virus structural proteins

US8460913B2 · kind B2 · utility

21Cited by

57References

6Claims

0Family size

Assignee

ALPHAVAX, INC. · US

Inventors

Kurt Iver Kamrud · Apex, US
Jonathan F. Smith · Cary, US
Maureen Maughan · Durham, US

Key dates

Filing date	Jun 20, 2008
Grant date	Jun 11, 2013
Priority date	—
Expiry date	Jun 16, 2030

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12N2770/36143
WIPO fieldPharmaceuticals
WIPO sectorChemistry

Abstract

The present invention provides an isolated RNA molecule comprising: a) an alphavirus 5′ replication recognition sequence, wherein at least one initiation codon has been removed from the 5′ replication recognition sequence; b) a nucleotide sequence encoding an alphavirus structural protein; and c) an alphavirus 3′ replication recognition sequence, with the proviso that the RNA molecule does not contain a promoter that directs transcription of the nucleotide sequence of (b), and wherein the alphavirus 5′ and 3′ replication recognition sequences of (a) and (c) direct replication of the RNA molecule in the presence of alphavirus non-structural proteins.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.