Methods and kit for isolating nucleic acids

US8460941B2 · kind B2 · utility

Assignee

• QIAGEN GmbH · DE

Inventors

• Christoph Ritt · Rahden, DE
• Christoph Erbacher · Haan, DE
• Patrick BAUMHOF · Dußlingen, DE

Key dates

• Filing date: May 7, 2012
• Grant date: Jun 11, 2013
• Expiry date: May 7, 2032

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10T436/255
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention is related to a method for isolating a target nucleic acid from a sample comprising said target nucleic acid, comprising the steps of mixing a sample containing said target nucleic acid with a binding solution and a nucleic acid binding matrix, binding at least part of said target nucleic acid to said nucleic acid binding matrix, wherein said nucleic acid binding matrix is treated simultaneously or has been previously treated with at least one compound comprising a metal substance selected from the group consisting of metals of the main groups 13 to 16, semimetals and transition metals for reducing non-target nucleic acid contaminations or wherein said nucleic acid binding matrix is modified with hydrophobic groups. Furthermore, respective kits and reagents are provided with the teaching of the present invention.